Table 2.

Primer and Probe Sequences of the Cytokines and the Housekeeping Gene Designed Using Primer Express Software

Gene	Primer and probe
IL-2	S: 5′- AAG AAT CCC AAA CTC ACC AGG AT -3′
	A: 5′- TGC TGA TTA AGT CCC TGG GTC TTA -3′
	P: 5′- (FAM)- CAT GCC CAA GAA GGC CAC AGA ACT G -(TAMRA) 3′
IL-4	S: 5′- ACA GGC ACA AGC AGC TGA TC -3′
	A: 5′- CCT TCA CAG GAC AGG AAT TCA A -3′
	P: 5′- (FAM)- TGA AAC GGC TCG ACA GGA ACC TCT G -(TAMRA) -3′
IL-5	S: 5′-CTT GGA GCT GCC TAC GTG TAT G -3′
	A: 5′- ATG TAC AGG AAC AGG AAT CCT CAG A -3′
	P: 5′- (FAM)- CCC CAC AGA AAT TCC CAC AAG TGC A -(TAMRA) -3′
IL-10	S: 5′- ATG AAG GAT CAG CTG GAC AAC TT -3′
	A: 5′- CCT TGA TGT CTG GGT CTT GGT -3′
	P: 5′- (FAM)- ACC TGG GTT GCC AAG CCT TGT CTG -(TAMRA) -3′
IFN-γ	S: 5′- GAA ACG AGA TGA CTT CGA AAA GC -3′
	A: 5′- CGA CCT CGA AAC AGC ATC TG -3′
	P: 5′- (FAM)- CCA AGT GAT GGC TGA ACT GTC GCC-(TAMRA) -3′
HPRT	S: 5′- GGC AGT ATA ATC CAA AGA TGG TCA A -3′
	A: 5′- GTC TGG CTT ATA TCC AAC ACT TCG T -3′
	P: 5′-(FAM)-CAA GCT TGC TGG TGA AAA GGA CCC C -(TAMRA) -3′

S, sense; A, antisense; P, probe.