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. 2005 Jan;166(1):303–312. doi: 10.1016/S0002-9440(10)62254-7

Figure 1.

Figure 1

Comparison of CARP transcription and translation levels between intact skin and excisional wounds in mice by real-time RT-PCR and Western blot. A: Time course of CARP mRNA levels in normal mice after wounding. Real-time, quantitative RT-PCR data showed that CARP transcripts were dramatically up-regulated during the first day after wounding and maintained increased expression by 25- to 35-fold from 3 days to 14 days. Each PCR reaction was measured in triplicate. B: Western blot analysis of CARP protein abundance during wounding. Fifty μg of lysate protein from mouse skin was taken at the indicated time points after excisional wounding and fractionated by SDS-PAGE. Top, signal detected with an anti-mouse CARP antibody; bottom, signal from an anti-β-actin antibody. C: Band intensities of the CARP signal assessed by densitometry after normalization for loading with β-actin values.