Figure 1.
Genotype determination by PCR using mouse tail genomic DNA and a specific primer pair designed from the MMP-9 gene exon 2 sequence, which was knocked out in BKO mice. The PCR products were analyzed by 1.5% agarose gel electrophoresis. A 225-bp band was generated from all six WT mice (left, lanes 1 to 6) tested but not from six BKO mice (right, lanes 1 to 6).