Figure 1.

Ocular regions used in this study. A: Color funduscopic photograph of the left eye of a middle-aged individual without any clinical signs of AMD. The macula (dashed circle) is centered on the fovea (arrow) and lies within the major retinal vessels (arrowheads) that emanate from the optic nerve head (ONH). The superior (S), inferior (I), temporal (T), and nasal (N) axes are marked for orientation. B: Diagrammatic representation of the distribution of the 2-mm-diameter retinal-choroidal-scleral punches collected from a 14-year-old donor. The solid black circle depicts the location of the optic nerve head, the curved black lines emanating from the optic disk represent the major retinal vessels, and the blue circle denotes the punch that was centered on the fovea. The remaining circles represent serial 2-mm-diameter punches taken along the superior, inferior, temporal, and nasal axes. C: Illustration showing the macular and extramacular (mid-peripheral) sites (circles) from which 4-mm-diameter punches were collected for EL thickness and integrity measurements in the large set of AMD and unaffected donors. Landmarks are the same as those described in B.