Table 6.

Semiquantitative IF Analysis of KC Gene Expression in the Oral Mucosa of Wild-Type and α3−/− Mutant Mice Exposed to ETS or Pure Nic

	α3+/+ mice			α3−/− mice
	Control	ETS	Nic	Control	ETS	Nic
p53	1.0	1.63 ± 0.22	1.48 ± 0.24	1.0	1.1 ± 0.11	1.12 ± 0.12
P		0.01	0.03		0.18	0.15
p21	1.0	1.62 ± 0.28	1.54 ± 0.25	1.0	0.97 ± 0.09	1.13 ± 0.19
P		0.02	0.02		0.62	0.31
Bcl-2	1.0	1.78 ± 0.22	1.74 ± 0.24	1.0	1.15 ± 0.17	0.99 ± 0.16
P		<0.01	0.01		0.2	0.89
Caspase-3	1.0	1.17 ± 0.12	1.19 ± 0.28	1.0	1.06 ± 0.32	0.87 ± 0.18
P		0.08	0.31		0.75	0.28
Filaggrin	1.0	2.05 ± 0.28	2.1 ± 0.29	1.0	1.07 ± 0.15	1.15 ± 0.21
P		<0.01	<0.01		0.45	0.27
NF-κB	1.0	2.6 ± 0.31	2.67 ± 0.11	1.0	1.16 ± 0.27	1.22 ± 0.32
P		<0.01	<0.01		0.35	0.31
JAK-1	1.0	1.12 ± 0.22	1.06 ± 0.26	1.0	1.03 ± 0.22	1.23 ± 0.23
P		0.4	0.73		0.81	0.17
STAT-1	1.0	3.06 ± 0.54	2.98 ± 0.38	1.0	1.15 ± 0.33	1.08 ± 0.21
P		<0.01	<0.01		0.48	0.58
GATA-3	1.0	1.79 ± 0.35	2.11 ± 0.25	1.0	2.12 ± 0.4	1.62 ± 0.2
P		0.02	<0.01		0.01	0.01
α5	1.0	2.04 ± 0.46	2.25 ± 0.16	1.0	1.06 ± 0.34	0.99 ± 0.41
P		0.02	<0.01		0.78	0.98
β2	1.0	2.14 ± 0.25	2.07 ± 0.45	1.0	1.1 ± 0.24	1.23 ± 0.49
P		<0.01	0.02		0.54	0.46
M2	1.0	0.39 ± 0.16	0.42 ± 0.22	1.0	1.05 ± 0.19	1.09 ± 0.53
P		<0.01	0.01		0.69	0.79
M3	1.0	0.36 ± 0.13	0.48 ± 0.04	1.0	1.11 ± 0.2	0.87 ± 0.39
P		<0.01	<0.01		0.4	0.6

Cryostat sections of oral mucosa were stained with the indicated antibodies (Table 4). The intensities of fluorescence produced by antibody binding to KCs in the samples from experimental mice are expressed relative to levels determined in KCs residing on the oral mucosa of intact wild-type mice (taken as 1). Data are means ± SD of at least two independent experiments. No specific staining could be seen in cryostat sections of murine oral mucosa in negative control experiments in which the primary antibody was either omitted or replaced with an irrelevant, isotype-matching antibody (not shown).