Table 7.
Summary of Changes of the KC Regulatory Gene Expression
| Experimental conditions | Regulatory genes
|
|||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| p53
|
p21
|
Bcl-2
|
Caspase 3
|
Filaggrin
|
NF-κB
|
JAK-1
|
STAT-1
|
GATA-3
|
||||||||||
| PCR | WB | PCR | WB | PCR | WB | PCR | WB | PCR | WB | PCR | WB | PCR | WB | PCR | WB | PCR | WB | |
| ETS + | ||||||||||||||||||
| HKC | ↑* | ↑ | ↑* | ↑ | ↑* | ↑ | ↑* | ↑ | ↑* | ↑ | ↑* | ↑* | ↑ | ↑ | ↑* | ↑* | ↑ | ↑* |
| −/−mice | ↑* | ↑* | ↑* | ↑* | ↑* | ↑* | ↑ | ↑ | ↑* | ↑* | ↑ | ↑ | ↑* | ↑ | ↑ | |||
| −/−MKC | ↑ | ↑ | ↑ | ↑* | ↑* | ↑* | ↑* | ↑* | ↑* | ↑* | ↑* | ↑* | ↑* | ↑* | ↑* | ↑* | ||
| Nic + | ||||||||||||||||||
| HKC | ↑* | ↑* | ↑* | ↑* | ↑* | ↑ | ↑ | ↑* | ↑* | ↑* | ↑* | ↑* | ↑ | ↑ | ↑* | ↑* | ↑ | ↑ |
| −/−mice | ↑* | ↑* | ↑* | ↑* | ↑* | ↑ | ↑* | ↑ | ↑* | ↑* | ↑ | ↑ | ↑* | ↑* | ↑* | ↑* | ||
| −/−MKC | ↑* | ↑* | ↑* | ↑* | ↑* | ↑* | ↑ | ↑* | ↑* | ↑* | ↑* | ↑* | ↑ | ↓* | ↑* | ↑* | ↑* | ↑ |
| siRNA-α3 | ↓* | ↓ | ↓* | ↓ | ↓ | ↓ | ↓* | ↓* | ↓ | |||||||||
ETS, environmental tobacco smoke; HKC, human keratinocytes; MKC, murine keratinocytes; Nic, nicotine; WB, Western blot.
*
P < 0.05, the rest are P > 0.05, compared to appropriate controls.