Bladder cells create distinct growth factor environments in conditioned media. Immunoassay results for HGF, TGFβ, VEGF, and bFGF are presented. A and B: Since levels of HGF and VEGF in control medium were nearly undetectable, these data are presented as concentration of rat growth factor level. C and D: The levels of TGFβ and bFGF are presented as percentage of control level (ie, 15% FBS), because considerable levels of these factors were also detected in control medium. These growth factors could be classified in three categories. A and C: 1) Growth factors that were predominantly present in SMC-CM (HGF and TGFβ. B and D: 2) Growth factors that were predominantly present in UC-CM (VEGF and bFGF). 3) Growth factors that remained below physiological levels (<20pg/ml, PDGF-BB and SCF).