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. 2005 Feb;166(2):565–573. doi: 10.1016/S0002-9440(10)62278-X

Figure 6.

Figure 6

Simulation of conditioned media by exogenous growth factors reproduced the phenotype change in marrow cells. Growth factors are added to αMEM + 0.5%FBS based on the ELISA data. A: Growth curve of MSC. MSC cultured in simulated UC-CM showed higher proliferation, which may be attributed to condensed cellular configuration. B: Immunofluorescence for SMC markers (magnification, ×400). The cells were fixed with 4% paraformaldehyde, incubated with αSMA and calponin antibodies (1:100 for each), and visualized with FITC-conjugated anti-mouse immunoglobulin. The nucleus was counterstained with Hoechst 33258 dye. C: Immunoblotting: simulated SMC-CM and UC-CM induced the similar differential phenotypic change in MSC.