Figure 8.

Hbi-pro-NGF forms separated by gel filtration chromatography induce cell death in 3T3-p75st cells. Three fractions of gel filtration chromatography of hbi-pro-NGF (8 μg total protein of each fraction) containing pro-NGF forms of 54, 32, and 26 kd, were added to 3T3-p75st for 30 hours. Apoptotic nuclei morphology was determined by Hoechst staining. Three μg of Hbi-pro-NGF was used as control of death induction in deprived cells. Apoptotic cell nuclei were counted as percentage of the total cells in each treatment. Bars are median ± SD of triplicates.