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. 2005 Feb;166(2):511–520. doi: 10.1016/S0002-9440(10)62273-0

Figure 2.

Figure 2

Real-time quantitative RT-PCR analysis of TWEAK and Fn14 mRNA expression in mouse brain after cerebral infarction. Mice were subjected to MCAO and then sacrificed at the indicated time points. RNA was isolated from the ipsilateral hemisphere (IH) and contralateral hemisphere (CH) regions dissected from the same brain specimen and real-time RT-PCR was performed to analyze TWEAK mRNA (A) or Fn14 mRNA (B) expression. TWEAK and Fn14 mRNA expression levels were normalized to GAPDH mRNA expression, and then the IH and CH 24-, 48-, and 72-hour values were normalized to the corresponding 0-hour value. The values shown are mean ± SEM from one cDNA preparation analyzed in triplicate. Similar results were obtained with a second cDNA preparation. *, P < 0.05 versus the corresponding 0-hour value.