Figure 3.

A: Effects of hypoxia on Epo secretion by melanoma. Tumor cells were incubated in normoxic and hypoxic conditions; the conditioned medium was collected and concentrated. β-Actin in the medium was used as a loading control. Representative blots from at least three independent experiments. Epo secretion was significantly increased under hypoxic conditions. B: Effects of CoCl₂ on Epo secretion by melanoma. Representative blots from at least three independent experiments. β-Actin in the medium was used as a loading control. Epo secretion was significantly increased after treatment with CoCl₂.