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. 2005 Mar;166(3):695–708. doi: 10.1016/s0002-9440(10)62291-2

Figure 5.

Figure 5

Elevated numbers of wound-healing hepatic myofibroblasts in nfκb1−/− livers. A: Hepatic α-SMA immunostaining at days 1 and 7 after injury. Photomicrographs are representative of four nfκb1−/− and five nfκb1+/+ mice at each time point. B: Number of α-SMA-positive cells were counted in 15 high-power fields and expressed as average number of α-SMA-positive cells/high-power field ± SEM from four nfκb1−/− and five nfκb1+/+ animals at each time point. Day 1, P = 0.0017; day 3, P = 0.035; day 7, P = 0.048; and day 14 is not significant; P values calculated using a paired t-test. C: TaqMan quantification of α-SMA mRNA measured in 20 ng of cDNA from whole liver at days 1, 3, 5, 7, and 14 of recovery. The relative level of transcriptional difference was expressed as an average ± SEM from four nfκb1−/− and five nfκb1+/+ animals per time point. Average cycle numbers were: day 1, 32.42 and 33.57; day 3, 33.66 and 35.16; day 7, 35.29 and 36.69; day 14, 35.62 and 37.08 for nfκb1−/− and nfκb1+/+, respectively. However, differences were not found to be statistically significant, P = 0.16, using the paired t-test. Original magnifications, ×200.