Skip to main content
. 2005 Mar;166(3):695–708. doi: 10.1016/s0002-9440(10)62291-2

Figure 8.

Figure 8

Elevated TNF-α in chronic injured nfκb1−/− liver. A: Total RNA was isolated from the livers of three nfκb1−/− and three nfκb1+/+ mice at day 1 after 12 weeks after injury and were used as templates in RT-PCR reactions for TNF-α and β-actin transcripts. The gels show RT-PCR products for the three livers/genotype and were representative of two repeat RT-PCRs for each RNA sample. B: Whole liver protein extracts were at day 1 after injury and sandwich ELISA was used to quantify TNF-α (as average ng of TNF-α/μg whole liver protein ± SEM, n = 4 nfκb1−/− and 5 nfκb1+/+). C: TNF-α immunostaining on liver sections at day1 after injury, arrows denote TNF-α-expressing cells mainly localized to regions surrounding inflammatory infiltrates in nfκb1−/− livers. Photomicrographs are representative of four nfκb1−/− and five nfκb1+/+ animals. D: TNF-α and α-SMA dual immunostaining on nfκb1−/− liver sections at day1 after injury, yellow arrows denote TNF-α and α-SMA co-localization. Original magnifications: ×100 (C, top); ×200 (C, bottom); ×1000 (D).