Figure 4.

Role of Tax and NF-κB activation on EBI3 expression by HTLV-1-infected cells. A: Jurkat cells were transfected with pJFE control vector (lane 1) or the indicated amount (in μg) of pJFE-Tax (lanes 2 to 4) or pJFE-M22 (lanes 5 and 6). The total amount of plasmid DNA transfected was kept constant in each transfection by addition of pJFE vector. Cells were lysed 22 hours after transfection in Nonidet P-40 lysis buffer and cell lysates (10⁶ cells per lane) were analyzed by immunoblotting with anti-EBI3 and anti-Tax antibodies. B: HUT-102 cells were treated with 5 μmol/L BAY 11-7082 (+) or with dimethyl sulfoxide (−). At time indicated at the top of the blot, cells were lysed in Nonidet P-40 lysis buffer and cell lysates (60 μg per lane) were analyzed by Western blot for EBI3 and Tax expression.