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Immunohistochemical analysis of a necrotic area of breast cancer sample using an antibody raised against the human HMGB1 protein. a: HMGB1 is present in the nuclei and cytoplasm of the breast cancer cells next to the necrotic area. b: Magnification of boxed area of a. HMGB1 is detectable in the nuclei as well as in the cytoplasm of macrophages. Original magnifications: ×100 (a); ×400 (b).
