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Smad2 and Smad3 are phosphorylated not only at C-terminal regions but also at linker regions in the activated HSCs after CCl4 intoxication. HSCs were obtained from normal rat liver and injured livers at 36 and 72 hours after CCl4 intoxication. Cell lysates were subjected to anti-Smad2/3 immunoprecipitation (IP), and were immunoblotted with each anti-pR-Smad Ab (top). Relative amounts of endogenous Smad2/3 were determined by immunoblotting (IB) using anti-Smad2/3 Ab (bottom).
