Table 1.
Effect of Capsaicin Treatment on the Expressions of Different Markers on ORS Keratinocytes
| Marker | Capsaicin (% of control) |
|---|---|
| Proliferation | |
| Ki-67 | 73.8 ± 7* (↓) |
| PCNA (proliferating cell nuclear antigen) | 71.6 ± 9* (↓) |
| Apoptosis | |
| Annexin-V | 143 ± 8* (↑) |
| Differentiation | |
| CK-14 (cytokeratin-14) | 94.2 ± 11 |
| CK-17 (cytokeratin-17) | 98.3 ± 6 |
| Filaggrin | 102.3 ± 8 |
| Involucrin | 95.1 ± 13 |
| Hair growth-promoting factors | |
| HGF (hepatic growth factor) | 63.5 ± 81* (↓) |
| IGF-I (insulin-like growth factor-I) | 53 ± 9* (↓) |
| SCF (c-kit/stem cell factor) | 74.9 ± 6* (↓) |
| Hair growth-inhibitory factors | |
| FGF-5 (fibroblast growth factor-5) | 95 ± 5 |
| IFN-γ (interferon-γ) | 91.3 ± 9 |
| IL-1β (interleukin-1β) | 143.7 ± 5* (↑) |
| TGF-β2 (transforming growth factor-β2) | 128.6 ± 6* (↑) |
| TNF-α (tumor necrosis factor-α) | 98.6 ± 4 |
Flow cytometry analyses were performed, as described under Materials and Methods, on control and capsaicin-treated (10 μmol/L for 2 days in high-Ca2+ SFM) ORS keratinocytes, and values of the treated samples were normalized as percentage of the control regarded as 100%. Data are expressed as mean ± SEM of three determinations.
*
Significant (P < 0.05) alterations.