Inflammatory cells in T/R+ and T/R− spinal cords. SCI site (T7 to T9) and lumbar region sections were immunostained for detection of Mac-3 (C, D, G, and H) and F4/80 (A, B, E, and F), 8 days after SCI in T/R+ (B, F, D, and H) and T/R− (A, E, C, and G). A–D: The injury site. E–H: Lumbar area, distant from the SCI site. All slides were stained and color was developed in parallel. The injury site of SCI-subjected T/R− mice displays enhanced Mac-3 and F4/80 reactivity compared to T/R+ mice. In the lumbar region, the presence of F4/80 and Mac-3+ cells was comparable and low. Sections are representative of four animals per group. Original magnifications: ×16 (A–D); ×10 (E–H).