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. 2005 Jun;166(6):1883–1894. doi: 10.1016/s0002-9440(10)62497-2

Figure 4.

Figure 4

αvβ8-mediated activation of TGF-β by astrocytes inhibits endothelial migration in fibrin gels. A polyoma middle T-transformed murine brain endothelial cell line (bEND.3) was retrovirally transduced with a retrovirus encoding the enhanced green fluorescence protein (GFP) and was allowed to form a confluent monolayer on porcine collagen-coated microcarrier beads. Endothelial cell-coated beads alone (A, B, G, H, M, N, S, T), or endothelial cell-coated beads co-cultured with astrocyte-coated beads (C, D, I, J, O, P, U, V), or astrocyte-coated beads alone (E, F, K, L, Q, R, W, X) were embedded into fibrin gels containing either no additions (A–F), recombinant active TGF-β (G–L), anti-TGF-β1 (M–R), or anti-β8 (S–X). The assay was allowed to proceed 48 hours. The endothelial cells are phase and GFP-bright and the astrocytes are phase-bright and GFP-negative. Shown are representative phase (A, C, E, G, I, K, M, O, Q, S, U, W) and fluorescent (GFP) fields (B, D, F, H, J, L, N, P, R, T, V, X).