Figure 7.

Cells lavaged from TRAF1−/− mice produced more TNF-α than did WT cells. Cells obtained from BAL of WT (gray bars) or TRAF1−/− (black bars) mice 20 hours after intratracheal treatment with saline or rmTNF-α were cultured in brefeldin A and RPMI media for 4 hours before harvest in anti-protease buffer. Murine TNF-α was assayed by ELISA in conditioned media (A) and cell pellet homogenates (B). The data are presented relative to the total protein concentration of the cell pellets. Mean ± SEM, n = 6 to 7 mice. *, P < 0.05 TRAF1−/− versus WT.