Figure 1.
Picrosirius red stain for mature collagen reveals significant deposition in lean, but not fa/fa, rats subjected to BDL. Picrosirius red stain demonstrating collagen deposition principally found around supporting structures (A–C, black arrows). D: Representative image revealing collagen deposition (black arrows) as well as extensive bridging fibrosis (blue arrow) in transition to cirrhosis. E: Histogram representing image quantitation of the mean percent collagen fibers/total slide area scanned in μm2± SEM determined as described in Materials and Methods. *P < 0.05, compares lean BDL versus sham-operated or fa/fa BDL rats. Original magnification, ×10 (D).