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. 2005 Jun;166(6):1647–1654. doi: 10.1016/S0002-9440(10)62475-3

Figure 7.

Figure 7

Reduced number of M cells within the FAE of CCR6-deficient mice. M cells were identified by staining with the rhodamine-conjugated UEA-1 lectin known to specifically label M cells inside the murine FAE. A: A typical staining of an M cell (red) that contains several leukocytes in its basolateral pocket. B: To analyze the effect of CCR6 on M-cell formation, multiple sections of Peyer’s patch of heterozygous control and CCR6-deficient mice from multiple litters were stained. The total number of follicle-associated epithelial cells was quantified by nuclear counterstain (DAPI, blue). Whereas control mice (open bars) contain 6.8% M cells within the FAE, CCR6 KO mice (filled bars) exhibited a significantly reduced number (1.8%) of UEA-1+ cells inside FAE (P < 0.001; C). The data represent the mean and SEM from 10 sections of six randomly selected Peyer’s patches of five mice representing each genotype.