Figure 3.

Copper incorporation into SOD1 is impaired in CCS knockout mice. Protein extracts from various tissues of wild-type, heterozygous, and homozygous CCS knockout mice and homozygous SOD1 knockout mice were obtained. (a) SDS/PAGE gel of liver (50 μg), kidney (75 μg), brain and spinal cord (100 μg), immunoblotted with antisera to SOD1. (b) SOD1 gel activity assay on same samples as a. (c) ⁶⁴Cu incorporation into SOD1, 100 μg of protein lysate for each tissue. (d) Analysis of serum samples from wild-type, heterozygous, and homozygous CCS knockout mice and homozygous ceruloplasmin knockout mice. (Top to Bottom) Immunoblot with antisera to human ceruloplasmin, ⁶⁴Cu incorporation to ceruloplasmin, and ceruloplasmin activity gel assay.