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. 2000 Feb 29;97(6):2892–2897. doi: 10.1073/pnas.050004797

Figure 5.

Figure 5

Fibrillar Aβ deposits and neuritic plaques develop in the presence of human apoE. Coronal sections through the hippocampus of an APPV717F +/−, apoE4+/− (A, B, D, and E) and an APPV717F +/−, apoE−/− mouse at 15 months of age were stained with thioflavine-S (A, B, and C) or the de Olmos silver degeneration method. Thioflavine-S-positive plaques were observed in the molecular layer of the dentate gyrus (A) and corpus callosum (B) of this apoE4-expressing mouse whereas no thioflavine-S-positive deposits were found in apoE−/− mice (C). In addition to fibrillar Aβ, neuritic plaques also were found in the hippocampus of apoE4-expressing mice (D and E). [Bar in C = 180 μm (A–D); bar in E = 30 μm (E).] (F) Representative Western blot analysis of apoE, APP, and LRP in hippocampal lysates of APPV717F +/− TG mice expressing apoE3 (line 37) or apoE4 (line 22) at 15 months of age. There were no significant differences in the level of apoE, APP, or LRP between the apoE3- and apoE4-expressing mice, as shown here qualitatively or quantitatively assessed by densitometric analysis (data not shown).