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. 2005 Jul;167(1):27–37. doi: 10.1016/s0002-9440(10)62950-1

Figure 1.

Figure 1

Ad-IκB suppresses LPS-induced nuclear translocation of NF-κB. BMDMs were transduced with relevant adenoviral vectors and stimulated 48 hours later with either LPS or a combination IFN-γ/LPS. Nuclear extracts were removed and analyzed for the presence of NF-κB using EMSA. Identity of NF-κB was assessed by a supershift (SS) in the NF-κB molecule that resulted from the incubation of cell extract from LPS-stimulated cells with 2 μg/ml of rabbit polyclonal antibody directed against the N-terminal of p65 subunit and by transduction with Ad-p65RHD, which produced a prominent nuclear band.