Table 1.

Properties of Ad-IκB-Transduced Macrophages

Pfu/cell	Percentage of cells transduced	Mean fluorescent intensity	Viability
50	64 ± 4	122	99 ± 8
100	89 ± 6	143	100 ± 6
150	86 ± 8	137	98 ± 11
200	72 ± 5	149	89 ± 7

Transduction efficiency, mean fluorescence intensity, and viability of Ad-IκB-transduced bone marrow-derived macrophages as determined by flow cytometry using an anti-rat IκB antibody and by propidium iodide, respectively. Maximum transduction efficiency with minimum cell death was achieved with 100 pfu/cell.