Figure 2.

S2-16:IFA-primed splenocytes proliferated to S2-16 and produced low levels of IFN-γ and IL-2 but high levels of IL-10. Lewis rats were treated with S2-16:IFA, and S2-16:CFA or PBS:CFA as controls. Fourteen days later, splenic lymphocytes were collected and cultured with S2-16 peptide to determine their in vitro recall response. A: Proliferative response of splenocytes after culture with different concentrations of S2-16. Proliferation was measured by ³H-thymidine incorporation. B: Cytokine production of splenocytes immunized in vivo and treated in vitro as indicated in figure and in legend above. Cell culture supernatants were collected for measurement of IFN-γ, IL-2, and IL-10 by ELISA. In some experiments, recombinant murine IL-12 or anti-rat IL-10 monoclonal antibody were added together with S2-16 to the cell culture as described in Materials and Methods. Error bars represent SEMs, and Student’s t-test was used to determine the significant differences between PBS:CFA-treated group and S2-16:IFA- or S2-16:CFA-treated group (*P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.001).