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. 2005 Jul;167(1):5–15. doi: 10.1016/S0002-9440(10)62948-3

Figure 3.

Figure 3

S2-16 and adjuvant treatment affected cytokine production of DCs. TNF-α production was increased in S2-16:CFA DCs as well as IL-12p40 mRNA, whereas increased IL-10 production was observed for S2-16:IFA DCs. Lewis rats were injected with S2-16:IFA or S2-16:CFA as a control, or left untreated. Fourteen days later, DCs were isolated from spleens by positive selection using OX62 monoclonal antibody and were stimulated by LPS or CpG-containing oligonucleotide with anti-rat CD40 antibody. TNF-α and IL-10 production from DCs in culture supernatants were measured by ELISA. IL-12p40 mRNA expression in rat DCs were measured by quantitative real-time PCR. Rat G3PDH was used as an endogenous control to allow for relative mRNA quantification. Cytokine mRNA levels are presented as fold increase in gene expression observed in triplicate wells of LPS and anti-CD40 antibody-treated DCs relative to untreated DCs. Error bars represent SEMs, and Student’s t-test was used to determine the significant differences between DCs from untreated rats and DCs from S2-16:IFA- or S2-16:CFA-treated rats (*P ≤ 0.05, **P ≤ 0.005).