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. 2005 Jul;167(1):89–95. doi: 10.1016/S0002-9440(10)62956-2

Figure 1.

Figure 1

Phenotype, genotype, and mRNA expression of transgenic mice. A: Gross eye phenotype. Narrow palpebral fissure (blepharophimosis), thickened eyelid margins, and missing hair around eyelashes, but normal eyelashes in Tabby male mice (TA). Note white (arrow) and red corneal opacities (insert, also mucus) in Tabby (TA). mEDA-A1 transgene (TATG) almost fully rescued Tabby phenotype. Note “yellow hair ring” around eyelids in TATG and wild-type transgenic (WTTG) mice. B: Genotyping of transgenic mice. Lane 1, 390-bp PCR product from endogenous genomic EDA; lane 2, 330 bp of PCR product only from transgene; lane 3, SSCP for genetic background detection. PCR product from Tabby is 1 bp shorter than wild type, so that it moves faster on gel electrophoresis. C: Expression level of EDA in mice. Note lower EDA expression in Tabby (TA) and very high expression in transgenic mice (TATG and WTTG).