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. 2005 Aug;167(2):545–554. doi: 10.1016/S0002-9440(10)62996-3

Figure 2.

Figure 2

Infiltration of macrophages/microglia in EAE. Spinal cords were removed at 17 to 20 dpi and at day 35 dpi onwards of EAE and cut longitudinally. Frozen sections were stained with an antibody against F4/80 (A–I), CD45 (J–L), and CD4 (not shown) to assess the infiltration/migration of inflammatory mononuclear cells. In control animals there are no mononuclear cells in the spinal cord tissue (not shown). There is a delay in microglial migration and infiltration of macrophages and lymphocytes in uPAR−/− mice (C) when compared to WT and tPA−/− animals at 20 dpi (A and B). F: However, perivascular cuffs containing mononuclear cells are evident in uPAR−/− mice at the peak of disease, 35 dpi. A high degree of persisting inflammation is evident in uPAR−/− mice at 60 dpi (I) although not in WT animals (G). J–L: CD45 showed very similar patterns of staining to F4/80. Original magnifications: ×100 (A–L); ×400 (insets).