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The protein bands recognized by antibodies to ARs in rat kidney homogenates. Kidney homogenates were prepared and the equivalent of 50 μg (for analysis of A1-AR and A2b-AR) or 80 μg (for analysis of A2a-AR and A3-AR) protein was subjected to Western blot analysis (lane 1) as described in Materials and Methods. On lane 2 blots performed in the presence of respective blocking peptides are shown.
