Figure 3.
Cellular distribution of adenosine A1 receptor in normal and diabetic kidney. Membrane (A) and cytosolic (B) fractions from whole normal kidney (KN), whole diabetic kidney (KD), normal kidney cortex (CN), diabetic kidney cortex (CD), normal kidney medulla (MN), and diabetic kidney medulla (MD) were prepared as described in Materials and Methods. The proteins (30 μg and 40 μg of membrane and cytosolic protein, respectively) were separated on 12% SDS-PAGE and immunoblotted with appropriate antibodies. The blots were scanned and quantified. C: The quantified results normalized to appropriate reference protein are presented as percentage of A1-AR/(reference protein) measured in normal (control) tissues + SD of three experiments. *P < 0.05 relative to control.