Figure 4.
Adenosine A2a receptor mRNA and protein levels in kidney of normal and diabetic rat. A: Total RNA was extracted from whole normal kidney (KN), whole diabetic kidney (KD), normal kidney cortex (CN), diabetic kidney cortex (CD), kidney cortex prepared from diabetic rats receiving insulin for 4 days (CD+I), normal kidney medulla (MN), diabetic kidney medulla (MD), and kidney medulla prepared from diabetic rats receiving insulin for 4 days (MD+I). The level of adenosine A2a receptor (A2a-AR) mRNA was analyzed as described in Materials and Methods. The results normalized to β-actin are presented as percentage of A2a-AR mRNA measured in normal (control) tissues + SD of at least five experiments. *P < 0.05 relative to control. B: Homogenates were prepared from analyzed tissues, and the equivalent of 80 μg of protein was subjected to Western blot analysis. The presented blot is representative of those obtained in three independent experiments. Protein p14-3-3 was used as a reference. The blots were scanned and quantified. The quantified results normalized to protein p14-3-3 are presented as percentage of A2a-AR/p14-3-3 measured in normal (control) tissues + SD of three experiments. *P < 0.05 relative to control.