Figure 6.
Adenosine A2b receptor mRNA and protein levels in kidney of normal and diabetic rat. A: Total RNA was extracted from whole normal kidney (KN), whole diabetic kidney (KD), normal kidney cortex (CN), diabetic kidney cortex (CD), kidney cortex prepared from diabetic rats receiving insulin for 4 days (CD+I), normal kidney medulla (MN), diabetic kidney medulla (MD), and kidney medulla prepared from diabetic rats receiving insulin for 4 days (MD+I). The level of adenosine A2b receptor (A2b-AR) mRNA was analyzed as described in Materials and Methods. The results normalized to β-actin are presented as percentage of A2b-AR mRNA measured in normal (control) tissues + SD of at least four experiments. *P < 0.05 relative to control. B: Homogenates were prepared from analyzed tissues, and the equivalent of 50 μg of protein was subjected to Western blot analysis. The presented blot is representative of those obtained in three independent experiments. β-Actin was used as a reference protein. The quantified results of Western blot are presented as percentage of A2b-AR/β-actin ratio measured in normal (control) tissues + SD of at least three experiments. *P < 0.05 relative to control.