Table 3.
Enzyme | Normal kidney (nmol/minute/mg)
|
Diabetic kidney (nmol/minute/mg)
|
||
---|---|---|---|---|
Cortex | Medulla | Cortex | Medulla | |
Nonspecific phosphohydrolases | 55.3 ± 13.2 | 29.3 ± 9.7 | 106.9 ± 18.9* | 59.7 ± 15.7* |
Ecto 5′-NT | 39.5 ± 9.1 | 6.1 ± 2.2 | 57.1 ± 14.2 | 27.3 ± 8.5* |
ADA | 17.2 ± 3.5 | 7.4 ± 2.6 | 26.7 ± 5.1* | 19.8 ± 4.3* |
The enzymatic activities were assayed in kidney membrane fractions of normal and diabetic rats in the presence of 100 μmol/L AMP as described in Materials and Methods. The values are mean + SD (n = 7).
P < 0.05 versus normal rats.