Table 3.

The Nonspecific Phosphohydrolase, Ecto 5′-Nucleotidase (Ecto 5′-NT), and Adenosine Deaminase (ADA) Activities in Kidney Membrane Fractions

Enzyme	Normal kidney (nmol/minute/mg)		Diabetic kidney (nmol/minute/mg)
	Cortex	Medulla	Cortex	Medulla
Nonspecific phosphohydrolases	55.3 ± 13.2	29.3 ± 9.7	106.9 ± 18.9*	59.7 ± 15.7*
Ecto 5′-NT	39.5 ± 9.1	6.1 ± 2.2	57.1 ± 14.2	27.3 ± 8.5*
ADA	17.2 ± 3.5	7.4 ± 2.6	26.7 ± 5.1*	19.8 ± 4.3*

The enzymatic activities were assayed in kidney membrane fractions of normal and diabetic rats in the presence of 100 μmol/L AMP as described in Materials and Methods. The values are mean + SD (n = 7). 

^*P < 0.05 versus normal rats.