Figure 5.

Localization of c-jun and c-fos in ocular tissue. Pterygia (A–H) and normal conjunctiva (I–P) were serially sectioned and stained for c-jun (A, E, I, and M), c-fos (B, F, J, and N), and Sp1 (C, G, K, and O). Control reactions included omitting the primary antibody (H and P) or applying a relevant isotype control IgG antibody (D and L). Tissue sections in A to D and I to L were derived from one donor and tissue in E to H and M to P were derived from a second patient. Red staining denoted positive immunoreactivity. To illustrate the extent and location of the immunoreactivity, sections in A to C, E to G, I to K, and M to O were not counterstained. Because no reactivity was observed in any of the control reactions (D, H, L, and P), these sections were counterstained with hematoxylin. This pattern of expression is representative of all diseased and normal tissue specimens analyzed. Original magnifications, ×500.