Table 1.
Primary Antibodies Used for Immunohistochemistry and Western Blotting
| Antibody | Source | Catalogue no. | Application | Dilution | Temperature/time |
|---|---|---|---|---|---|
| Primary antibodies | |||||
| p-p44/p42 (RaH) | CST | 9101 | WB, IHC | 1:1000, (1:30) | 4°C/16 hours |
| p44/p42 (RaH) | CST | 9102 | WB, IHC | 1:1000, (1:30) | 4°C/16 hours |
| p-p38 (RaH) | SCB | sc-7975 | WB | 1:500 | 4°C/16 hours |
| MMP-1 (MaH) | ICN | 63-178 | WB | 1:1000 | 22°C/1 hour |
| pEGFR (MaH) | CST | 2236 | IHC | 1:400 | 4°C/16 hours |
| c-fos (RaH) | SCB | sc-52 | IHC | 1:200 | 37°C/1 hour |
| c-jun (RaH) | SCB | sc-1694 | IHC | 1:200 | 37°C/1 hour |
| Sp1 (RaH) | SCB | sc-59 | IHC | 1:200 | 37°C/1 hour |
| EGF (RaH) | SCB | sc-275 | IHC | 1:25 | 4°C/16 hours |
| HB-EGF (GaH) | R&D | AF-259-NA | IHC | 1:60 | 4°C/16 hours |
| Rabbit IgG | SCB | sc-2027 | IHC | 1:25–1:200 | 37°C/16 hours |
| Mouse IgG | Dako | X0931 | IHC | 1:400 | 4°C/16 hours |
| Goat IgG | R&D | AB-108-C | IHC | 1:60 | 4°C/16 hours |
| Secondary antibodies | |||||
| SaRHRP | Dako | P0217 | WB | 1:2000 | 22°C/1 hour |
| RaMHRP | Dako | P0260 | WB | 1:2000 | 22°C/1 hour |
| GaRb | Dako | E0466 | IHC | 1:200 | 22°C/30 minutes |
| GaMb | Dako | E0433 | IHC | 1:200 | 22°C/30 minutes |
| RaGb | Dako | E0466 | IHC | 1:200 | 22°C/30 minutes |
Abbreviations used: CST, Cell Signaling Technology (GeneSearch, Arundel, Australia); GaH, goat anti-human; GaMb, biotin-conjugated goat anti-mouse; GaRb, biotin-conjugated goat anti-rabbit; ICN, ICN Biomedicals (Sydney, Australia); IHC, immunohistochemistry; MaH, mouse anti-human; R&D, R&D Systems (Minneapolis, MN); RaGb, biotin-conjugated rabbit anti-goat; RaH, rabbit anti-human; RaMHRP, horseradish peroxidase-conjugated rabbit anti-mouse; SC, Santa Cruz Biotechnology (Santa Cruz, CA); WB, (Western blotting); SaRHRP, horseradish peroxidase-conjugated swine anti-rabbit.