Immunoblot analysis of term placenta and cells. A: Protein lysates from three different term placentas and from resting and PHA-activated human PBMCs were electrophoresed and subjected to immunoblot analysis and chemiluminescence detection for B7-H2, B7-H3, and actin. B: Protein from three different preparations of term villous cytotrophoblast cell preparations, Jar, JEG-3, or HUVECs were analyzed for these proteins.