Immunohistochemical localization of B7 family proteins in the first trimester placenta. A, C, E, G, and I: Extravillous trophoblast cell columns. B, D, F, H, and J: Villous portions of the placenta. A, B: B7-H2; C, D: B7-DC; E, F: B7-H3; G, H: rabbit IgG control; I, J: goat IgG control. Two-headed arrows in A, C, and E bracket cell columns. Arrowheads in B and F show positively staining stromal cells. Arrows in B show positively stained villous cytotrophoblast cells. Double arrows in D and F bracket the villous cyto- and syncytiotrophoblast. CC, cell column. Original magnifications, ×400.