Figure 6.

Cell invasion in organotypic rat brain slice. A: U87 glioma cells stably expressing GFP were transfected with siRNAs directed against luciferase (control) or R-Ras, 2 days before implantation into the bilateral putamen on rat organotypic brain slice and observed at the indicated times. B: Invasion rate of U87 cells treated with the indicated siRNA constructs were calculated from z axis images collected by confocal laser-scanning microscopy; Bars, SE. *, P < 0.05. The mean value of invasion rates was obtained from six independent experiments. C: Invasion rates of U251 and T98G cells stably expressing GFP were co-transfected with EphB2 and R-Ras siRNA or control siRNA were calculated as same as above. Scale bar, 500 μm.