Figure 4.
CsA induced alterations in HK-2 F-actin cytoskeletal arrangement. Cells were grown to confluency on Falcon 8-chamber glass slides. Cells were cultured in the absence (A, C) and presence (B, D) of 4.2 μmol/L CsA for 48 hours. F-actin cytoskeletal arrangement was examined by immunofluorescent microscopy as described in the Materials and Methods section. One representative experiment of at least three individual replicate experiments is shown. Original magnifications: ×200 (A, B); ×630 (C, D).