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. 2005 Oct;167(4):1051–1059. doi: 10.1016/s0002-9440(10)61194-7

Figure 2.

Figure 2

Anti-Fas-induced apoptosis in HSY and Jurkat cells. A: The HSY and Jurkat cell apoptosis induced by anti-Fas mAb (CH-11) stimulation was determined by flow cytometry of DNA content of nuclei with PI and annexin V. B: Western blot analysis demonstrated 120-kd α-fodrin in apoptotic HSY and Jurkat cells in a dose-dependent manner. C: Effects of protease inhibitors on α-fodrin cleavage in apoptotic HSY cells. Proteolytic cleavage of α-fodrin to 120 kd in Fas-stimulated HSY cells is blocked by a combination of a calpain inhibitor peptide and caspase inhibitors (Z-VAD-fmk), but not by E64, leupeptin, and lepstatin. Calpain inhibitor peptide alone could not inhibit the 120-kd α-fodrin formation.