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. 2005 Oct;167(4):1093–1103. doi: 10.1016/S0002-9440(10)61198-4

Figure 6.

Figure 6

Evaluation of the role of EGF and IGF-1 on A+ cell growth. The cells were counted in a Neubauer chamber after 4, 7, 10, 15, and 21 days of culture. A: Expression of EGF receptor (EGFR) and IGF-1 receptor (IGF-1R) in the liver and angiomyolipoma of the TSC2 patient, and in A+ and VSMC cells. B: A+ cell growth in complete medium (containing 10 ng/ml EGF), in the same medium without EGF, and without EGF with the addition of IGF-1 50 or 5 ng/ml. The A+ cells proliferated and significantly increased in number only in the presence of EGF; IGF-1 failed to promote any increase. No reduction or mortality was observed under any of the experimental conditions. C: VSMC proliferation in specific standard medium, in the presence of 10 ng/ml EGF, and in the presence of 50 ng/ml IGF-1. IGF-1 supplementation led to a significant increase in cell number. Mean values ± SEM. Significant differences (*P < 0.05, ***P < 0.001) versus control were evaluated by Student’s t-test.