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. 2005 Oct;167(4):1093–1103. doi: 10.1016/S0002-9440(10)61198-4

Figure 7.

Figure 7

Western blot evaluation of the expression of Akt and its phosphorylated form on Ser473, S6K1, and its phosphorylated form on Thr389, 4EBP1, and its phosphorylated form on Thr37/46, the phosphorylated form of ERK on Thr202/Tyr204, and Ser2448-phosphorylated mTOR. A: The A+ cells were cultured for 21 days in standard medium, and in the same medium without EGF in the absence or presence of IGF-1 (50 ng/ml). The A+ cells were grown for 24 hours in serum-free medium without EGF and IGF-1 before S6K1 phosphorylation was evaluated (B), and incubated for 2 hours with IGF-1 before phospho-Erk was evaluated (C). The blots are representative of three experiments.