Figure 8.
Effect of antibodies (5 μg/ml) to EGF and IGF-1 receptors on A+ cell number. A total of 5 × 104 cells were plated and the antibodies were added to the complete medium (A) or to the same medium without EGF (B); the cells were then counted in a modified Neubauer chamber. There was a 50% reduction in cell number within 5 days, and complete cell loss in 12 days. Anti-IGF-1R and anti-EGFR clone EGFR.1 were apparently more effective than anti-EGFR clone 225, the difference being significant (P < 0.05) 5 days after plating. Mean values ± SEM. Significant differences (*P < 0.05, ***P < 0.001) versus control were evaluated by Student’s t-test.