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Analysis of the diaphragmatic rupture that occasionally occurs in E18.5 Lox−/− embryos. Masson’s trichrome staining shows that the rupture occurs at the collagen-rich diaphragmatic central tendon region (arrowheads in insets), thus allowing some of the abdominal contents to enter the thoracic cavity (arrows). L, liver; Lu, lung; H, heart; I, intestine.
