Figure 6.

Analysis of type I and IV collagen in E18.5 skin. a–d: Immunostaining of type I (a and b) and IV collagen (c and d) showed no obvious differences between the WT and Lox^−/− skin samples. e and f: Electron micrographs of the WT skin showed clearly defined, large bundles consisting of numerous individual collagen fibers (e), whereas the collagen bundles in the Lox^−/− skin were less well organized and contained fewer individual fibers (f). The collagen fibers generally had uncovered, clean surfaces in the WT skin (g), whereas the surfaces were surrounded by a precipitate-like material of undefined character in the Lox^−/− skin (h). Scale bars: 100 μm (a–d); 1 μm (e, f); 200 nm (g, h).