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. 2005 Oct;167(4):1061–1069. doi: 10.1016/S0002-9440(10)61195-9

Figure 6.

Figure 6

Effect of PGE2 on expression of MMP-9 and MMP inhibitors in peritoneal macrophages. Peritoneal macrophages were treated with 1 or 10 μmol/L PGE2. A: A representative gel picture showing RT-PCR result of levels of TIMP-1, TIMP-2, and RECK mRNA in macrophages. The experiment was repeated four times and the results were similar. B: A representative Western blot result demonstrates the expression of TIMP-1, TIMP-2, and β-actin protein. The experiment was repeated three times and the results were similar. C: A representative gel picture showing RT-PCR result of levels of MMP-9 mRNA in PGE2-treated macrophages. Expression of MMP-9 mRNA was decreased by treatment with 1 and 10 μmol/L PGE2, respectively. The experiment was repeated four times and the results were similar. D: Western blot analyses revealed a significant decrease in expression of MMP-9 protein with PGE2 treatment in peritoneal macrophages. The experiment was repeated four times and the results were similar.