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. 2005 Oct;167(4):1149–1159. doi: 10.1016/s0002-9440(10)61203-5

Figure 1.

Figure 1

Anti-sense oligonucleotides targeting PDGF-Rα block SMC growth in vitro. WKY12-22 SMCs were incubated overnight in 0.05% fetal bovine serum/Waymouth’s medium and transfected with 0.1 or 0.3 μmol/L PDGF-Rα AS ODN or its size-matched scrambled counterpart PDGF-Rα SCR ODN. The medium was changed to 0.05% fetal bovine serum/Waymouth’s and after 2 days the cells were trypsinized, resuspended in Isoton II, and quantitated using a Coulter counter. Endothelial cells (EC) grown in Dulbecco’s modified Eagle’s medium with the equivalent amounts of serum were treated identically. The data are representative of two experiments.