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. 2005 Oct;167(4):1149–1159. doi: 10.1016/s0002-9440(10)61203-5

Figure 3.

Figure 3

Ets-1 induces PDGF-Rα mRNA expression and transcription. A: Cells were transfected with 20 μg of pcDNA3-Ets-1 or pcDNA3 and PDGF-Rα or u-PA mRNA expressions were assessed by reverse transcriptase-polymerase chain reaction after 24 hours. The expected amplification product of PDGF-Rα is 1017 bp, GAPDH is 450 bp, and u-PA is 690 bp. Densitometric analysis was performed on PDGF-Rα, u-PA, and GAPDH mRNA bands. The result is representative of at least two separate determinations performed in duplicate. B: Ets-1 activates the PDGF-Rα promoter, whereas Fli-1 does not. SMCs were co-transfected with the PDGF-Rα promoter-reporter construct pLuc-a2, pRL-Null, and either pcDNA3-Ets-1 or pcDNA3-Fli-1. Luciferase activity was assessed by luminometry after 24 hours. C: Nucleotide sequence of the PDGF-Rα proximal promoter. The transcriptional (transc) start site (ATG)27 is indicated. The reverse Ets (−45TTCC−42) binding site, that is the subject of this study, and the upstream Sp1 element (−61/−52)24 are indicated. Oligo −80/−33 contains both putative Ets and Sp1 sites. The sequence was obtained from EMBL RN 13172 Rattus norvegicus.